Coding

Part:BBa_K2967009:Design

Designed by: Rui Wang   Group: iGEM19_NEU_CHINA   (2019-09-28)


The activator protein HrpS in hrp gene regulatory network


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 902
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 112
    Illegal SapI.rc site found at 745


Design Notes

We optimize the sequence to make it work well in E coli.



Source

It comes from the hrp gene regulatory network for Type III secretion in Pseudomonas syringae.

References